Catalog # SK6420
Semaphorin-3 and Neuropilin Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb |
 |
KIT SUMMARY The Semaphorin-3 and Neuropilin antibody sampler kit can be used to examine the expression of semaphorin 3A (Sema-3A) and 3F (Sema-3F), and their receptors, Neuropilin 1 (NRP1) and Neuropilin 2 (NRP2). Included in the kit are secondary reagents for detection of rabbit polyclonal and mouse monoclonal antibodies. |
Western blot analysis of Sema-3F expression in adult mouse brain (lane 1), kidney (lane 2), and liver (lane 3). The blot was probed with anti-Sema-3F (SP3921) at 1:1000.
Formalin fixed, citric acid treated parafin sections of adult Rat cerebellum. Sections were probed with anti-Sema3A (SP1221) then anti-Rabbit:HRP before detection using DAB. (Images provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
NP2111 Boro, A. et al. (2015) Am J Transl Res. 7(3):640-653 WB: human osteosarcoma cell linesRS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
NP2111 Ohkusu-Tsukada, K. et al. (2010) Int J Cancer. Epub 15 MAR. ELISA: sNRP1 mouse serum
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Neuropilin-1 (a1 CUB domain) Rabbit pAb | 50 μl | WB, E, ICC, IHC | Hu, Rt, Ms | 130 | |
| Neuropilin-2 (MAM domain) Mouse mAb | 50 μl | WB, E | Hu, Rt, Ms | 130 | |
| Semaphorin-3A (Central region) Rabbit pAb | 50 μl | WB, E, ICC, IHC | Hu, Rt, Ms | 95 | |
| Semaphorin-3F (a.a. 746-762) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 95 | |
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
