Catalog # EK6720

Estrogen Receptor Phospho-Regulation Antibody Kit

Antibody Sampler Kit



Estrogen receptor α (ERα) is a member of the steroid receptor superfamily and its structure includes an N-terminal ligand-independent transactivation domain (AF-1), a highly conserved DNA binding domain, and a C-terminal ligand-dependent transactivation domain (AF-2). AF-1 and AF-2 activate transcription independently and synergistically, and act in a promoter- and cell-specific manner. Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity. Ser-104, Ser-106, Ser-118, and Ser-167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. In addition to these sites, phosphorylation of Tyr-537 has been implicated in maximal hormone binding, dimerization, and transcriptional activity. Tyr-537, located in the AF-2 domain, is phosphorylated by c-Src leading to nuclear export of ERα and degradation. Thus, a variety of phosphorylation events control ERα activity.

Anbalagan M, Rowan BG (2015) Mol Cell Endocrin. 418(3):264.
Castoria, G. et al. (2012) Oncogene. 31:4868.

Western blot image of human MCF-7 cells treated with pervanadate (1 mM) for 30 min. (lanes 1-6). Some lanes of the blot were then treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with mouse monoclonal anti-ERα (Tyr-537) phospho-specific (lanes 1 & 2), rabbit polyclonal anti-ERα (C-terminus) (lanes 3 & 4), and rabbit polyclonal anti-ERα (Tyr-537) phospho-specific (lanes 5 & 6).

Immunocytochemical labeling of Estrogen Receptor α in paraformaldehyde fixed and NP-40 permeabilized MDA-MB-231 cells. The cells were labeled with rabbit polyclonal anti-Estrogen Receptor α (EP5431). The antibody was detected using goat anti-rabbit DyLight® 594.

Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot

This kit contains:

Estrogen Receptor α(C-terminus) Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms68
Estrogen Receptor α (Tyr-537), phospho-specific Rabbit pAb50 μlWB, EHu, Rt, Ms, Ck, Fr68
Estrogen Receptor α (Tyr-537), phospho-specific Mouse mAb 50 μlWB, EHu, Rt, Ms, Ck, Fr68
Anti-Mouse Ig:HRPDonkey pAb100 μlWB, EMs
Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb100 μlWB, E, ICC, IHCRb

The ERα Phospho-Regulation antibody sampler kit can be used to detect phosphorylation of Tyr-537 relative to total ERα expression levels. The kit includes mouse monoclonal and rabbit polyclonal antibodies to detect phospho-Tyr-537, as well as a rabbit polyclonal antibody to detect ERα. The kit also includes anti-Rabbit Light Chain specific:HRP and anti-Mouse Ig specific:HRP secondary reagents for detection of antibodies in Western blot, ELISA, or immunocytochemistry.