The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation of IκBα occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-κB. This pathway of IκBα regulation occurs in response to various NF-κB-activating agents, such as TNFα, interleukins, LPS, and irradiation. An alternative pathway for IκBα regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IκBα and NF-κB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IκBα nuclear translocation and inhibition of NF-κB-activation. Thus, tyrosine phosphorylation of IκBα may be an important regulatory mechanism in NF-κB signaling.
Bui, N.T. et al. (2001) J Cell Biol 152(4):753.
Waris et al. (2003) J Biol Chem 278(42):40778.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:IP1031 Cullen, S. et al. (2015) Biomolecules. 5(1):95 WB: ILU-18 cells
IP1041 Cullen, S. et al. (2015) Biomolecules. 5(1):95 WB: ILU-18 cells
IP1861 Karki, R. et al. (2014) Free Radic Biol Med. 71:256 WB: HEK-Blue mTLR4
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
IP1031 Wright, C.J. et al (2009) AJP Lung Cell Mol Phy 296(3):L296. WB: rat fibroblast
IP1031 Naidu, S. et al. (2008) J Immunol. 181:4113. WB: mouse RAW264.7
IP1031 Boosani, C.S. et al. (2007) Blood 110(4):1168. WB: mouse lung endothelial, MLEC
IP1031 Sethi, G. et al. (2007) Oncogene 26(52):7324. WB: human epithelial H1299, Y42F
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|IκBα (C-terminus) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||38|
|IκBα (Ser-32/Ser-36), phospho-specific Mouse mAb||50 μl||WB, E, IP||Hu, Rt, Ms||38|
|IκBα (Tyr-42) Rabbit pAb||50 μl||WB, E, IP||Hu, Rt, Ms||38|
|IκBα (Tyr-305), phospho-specific Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||38|
|Anti-Mouse Ig:HRP Donkey pAb||100 μl||WB, E||Ms|
|Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The IκBα phospho-regulation antibody sampler kit can be used to detect IκBα phosphorylation on Ser-32/Ser-36, Tyr-42, and Tyr-305. The kit also includes a polyclonal antibody to monitor total IκBα expression levels and secondary reagents for detection of these antibodies.