The ERK1/2 (p44/42) MAPK signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Upon stimulation, a sequential three-part MAP kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK), a MAP kinase kinase (MAPKK), and a MAP kinase (MAPK). Activation of the MAPKs, ERK1 and ERK2, leads to phosphorylation of activation loop residues Thr-202/Tyr-204 and Thr-185/Tyr-187, respectively. In addition to dual phosphorylation, ERK1 and 2 are autophosphorylated on Thr-207 or Thr-188, respectively. This phosphorylation is required for nuclear translocation of ERK, and leads to phosphorylation of several nuclear proteins involved in cardiac hypertrophy. Mouse models with mutation of Thr-188 in ERK2 show that this site is critical for ERK-mediated cardiac hypertrophy. Thus, phosphorylation of Thr-188 in ERK2 may be important for controlling the nuclear functions of activated ERK.
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Lorenz, K. et al. (2009) Nat Med. 15(1):75.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:EM2331 Kyjacova, L. et al. (2015) Cell Death Differ. 22(6):898. WB: human DU145
EP4101 H. Huang, et al. (2015) Cardiovasc Res. 108(1):50. WB: mouse heart
EM2061 Park, K. et al. (2013) Mol Cell Biol. 33(4):752. WB: human keratinocytes
EP4101 Lu, J. et al. (2013) Basic Res Cardiol. 108(2):326. WB fluorescence: mouse heart
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|ERK1 (C-terminal region) Mouse mAb||50 μl||WB, E, ICC, IHC||Hu, Rt, Ms||44|
|ERK1 (Thr-202/Tyr-204)[conserved] Mouse mAb||50 μl||WB, E, ICC||Hu, Rt, Ms||44|
|ERK2 (a.a. 181-195)[conserved site] Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms, Ck, F||42|
|ERK1 (Thr-207)/ERK2 (Thr-188)[conserved], phospho-specific Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms, Ck, F||42|
|Anti-Mouse Ig:HRP Donkey pAb||100 μl||WB, E||Ms|
|Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The ERK phospho-regulation antibody sampler kit can be used to examine ERK1 phosphorylation at Thr-202/Tyr-204 and Thr-207. These phosphorylation sites are also conserved in ERK2. A mouse monoclonal antibody to ERK1 and a rabbit polyclonal antibody to ERK2 are also included for examining total ERK expression levels.