Catalog # CK6120
β-Catenin Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, IP, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Ck, Fr |
 |
KIT SUMMARY The β-Catenin phospho-regulation antibody sampler kit can be used to examine phosphorylation of β-Catenin at Tyr-86, Tyr-142, Tyr-489 and Tyr-654. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of β-Catenin. |
Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).
Immunocytochemical labeling of β-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-86) or β-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
CP4021 Lu, Y. et al.(2014) Stem Cells Dev. 23(15):1755. IF: chicken embryonic fibroblastsCP1061 Kinoshita-Kikuta, E. et al. (2014) J Electrophoresis. 58(1) WB: SW480 and HEK293
CP1081 Tsuneki, M. et al. (2014) Mol Cell Biol. 34(24): 4485 IF: mouse EOMA, brain endothelial cells
CP4021 Condello, S. et al. (2013) FASEB Journal 27(8): 3100. WB: ovarian cancer cells
CM1181 Condello, S. et al. (2013) FASEB J 27(8):3100. WB & IHC: Ovarian Cancer Cells
CM1181 Yakubov, B. et al. (2013) Neoplasia 15(6):609. WB: Human Ovarian Cancer Cells
CP1081 Qi, F. et al. (2013) Am J Pathol. 183(5):1654. WB: human mesothelial
CP1081 Krejci, P. et al. (2012) PLoS ONE. 7(4):e35826. WB: rat chondrosarcoma cells
CP1061 Beard, R.S. et al. (2011) Blood. 118(7):2007. WB, ICC: mouse microvascular ECs
CP1081 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP1191 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Funakoshi S et al (2010) Am J Phys Gast Liv Phys 299(5):1054 WB: human colon cancer cells
CP1061 Harris, E.S. & Nelson, W.J. (2010) Mol Biol Cell. 21:2611. WB: HUVECs
This kit contains:
| CATALOG # | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| β-Catenin Mouse mAb | 50 μl | WB, E, IP, ICC, IHC | Hu, Rt, Ms | 92 | |
| β-Catenin (N-terminal) Rabbit pAb | 50 μl | WB, E, IP, IHC | Hu, Rt, Ms | 92 | |
| β-Catenin (Tyr-86), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 92 | |
| β-Catenin (Tyr-142)/[γ-Catenin (Tyr-133)], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 92 | |
| β-Catenin (Tyr-489)/[γ-Catenin (Tyr-480)], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 92 | |
| β-Catenin (Tyr-654)/[γ-Catenin (Tyr-644)], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, Fr | 92 |
