Catalog # CK6120

β-Catenin Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn Kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86, 489, and 654 in β-Catenin. Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin, while c-Abl phosphorylation of Tyr-489 decreases β-Catenin binding to N-Cadherin and leads to nuclear translocation and transcriptional activation.

References
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Rhee, J. et al. (2007) Nat. Cell Biol. 9(8):883.

Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489) conserved site (CP2961), or anti-β-Catenin (CM1181).

Immunocytochemical labeling of β-Catenin in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal β-Catenin (CM1181) or rabbit polyclonal β-Catenin (Tyr-86) or β-Catenin (Y654) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.



Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CP4021 Lu, Y. et al.(2014) Stem Cells Dev. 23(15):1755. IF: chicken embryonic fibroblasts
CP1061 Kinoshita-Kikuta, E. et al. (2014) J Electrophoresis. 58(1) WB: SW480 and HEK293
CP1081 Tsuneki, M. et al. (2014) Mol Cell Biol. 34(24): 4485 IF: mouse EOMA, brain endothelial cells
CP4021 Condello, S. et al. (2013) FASEB Journal 27(8): 3100. WB: ovarian cancer cells
CM1181 Condello, S. et al. (2013) FASEB J 27(8):3100. WB & IHC: Ovarian Cancer Cells
CM1181 Yakubov, B. et al. (2013) Neoplasia 15(6):609. WB: Human Ovarian Cancer Cells
CP1081 Qi, F. et al. (2013) Am J Pathol. 183(5):1654. WB: human mesothelial
CP1081 Krejci, P. et al. (2012) PLoS ONE. 7(4):e35826. WB: rat chondrosarcoma cells
CP1061 Beard, R.S. et al. (2011) Blood. 118(7):2007. WB, ICC: mouse microvascular ECs
CP1081 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP1191 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Funakoshi S et al (2010) Am J Phys Gast Liv Phys 299(5):1054 WB: human colon cancer cells
CP1061 Harris, E.S. & Nelson, W.J. (2010) Mol Biol Cell. 21:2611. WB: HUVECs

This kit contains:

CATALOG #DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
β-Catenin Mouse mAb 50 μlWB, E, IP, ICC, IHCHu, Rt, Ms92
β-Catenin (N-terminal) Rabbit pAb50 μlWB, E, IP, IHCHu, Rt, Ms92
β-Catenin (Tyr-86), phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms92
β-Catenin (Tyr-142)/[γ-Catenin (Tyr-133)], phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms92
β-Catenin (Tyr-489)/[γ-Catenin (Tyr-480)], phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms92
β-Catenin (Tyr-654)/[γ-Catenin (Tyr-644)], phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms, Ck, Fr92
KIT SUMMARY

The β-Catenin phospho-regulation antibody sampler kit can be used to examine phosphorylation of β-Catenin at Tyr-86, Tyr-142, Tyr-489 and Tyr-654. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of β-Catenin.