α-Actinins are widely expressed cytoskeletal proteins that cross-link actin filaments through antiparallel homodimers of the rod domains. Four α-actinin genes have been discovered in humans with α-actinin 1 and 4 being widely expressed in non-muscle cells. α-Actinins contain three conserved domains that include an N-terminal actin binding domain, four spectrin-like repeats in the central region, and a C-terminal calmodulin binding domain. α-Actinin cross-links the actin filament networks and associates the network to focal adhesion sites through binding of talin and vinculin. α-Actinin 1 is phosphorylated at Tyr-12 by FAK, while α-actinin 4 can be phosphorylated at Tyr-4 and Tyr-31 after EGF treatment. Tyr-4 and Tyr-31 phosphorylation inhibits actin binding and reduces actin-filament driven multinucleation in rat kidney cells. Thus, phosphorylation in α-actinins may be important for regulating actin binding and actin cytoskeletal remodeling.
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Shao, H. et al. (2010) J Biol Chem. 285(4):2591.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:AP4241 Kojima, S. et al. (2014) Am J Nephrol. 39(1):36-45 WB: human kidney cells
AP4271 Kojima, S. et al. (2014) Am J Nephrol. 39(1):36-45 WB: human kidney cells
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|α-Actinin 4 (a.a. 2-11) Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||104|
|α-Actinin 4 (a.a. 2-11) Peptide||50 μg||AB, E|
|α-Actinin 4 (Tyr-4), phospho-specific Rabbit pAb||50 μl||WB, E||Hu, Rt, Ms||104|
|phospho-α-Actinin 4 (Tyr-4) Peptide||50 μg||AB, E|
|Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The α-Actinin 4 phospho-regulation antibody sampler kit can be used to examine phosphorylation of α-Actinin 4 at Tyr-4. The kit includes polyclonal anti-α-Actinin 4 antibody to monitor total expression levels of α-Actinins, peptides for blocking experiments, and HRP-conjugated secondary reagent for primary antibody detection.