Catalog # CK6230


δ1-Catenin Phospho-Regulation

Antibody Sampler Kit

$395

Catenins are molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics wiith signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

References
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.
Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-δ1-Catenin (Tyr-96) (lanes 3 & 4), anti-δ1-Catenin (Tyr-228) (lanes 5 & 6), anti-δ1-Catenin (Tyr-280) (lanes 7 & 8) or anti-δ1-Catenin (Tyr-302) (lanes 9 & 10).

Immunocytochemical labeling of δ1-Catenin in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with rabbit polyclonal δ1-Catenin (Thr-916) (Left) or mouse monoclonal δ1-Catenin (a.a. 275-285) (Middle). Demonstration of the overlapping labeling patterns is shown on the right. The antibodies were detected using goat anti-rabbit DyLight® 488 or goat anti-mouse-DyLight® 594



This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
δ1-Catenin (a.a. 275-285) Mouse mAb50 μlWB, E, IP, ICCHu, Rt, Ms110
δ1-Catenin (Tyr-96), phospho-specific Mouse mAb 50 μlWB, EHu, Rt, Ms110
δ1-Catenin (Tyr-228), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms110
δ1-Catenin (Tyr-280), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms110
δ1-Catenin (Tyr-302), phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms110
δ1-Catenin (Thr-916), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms110
KIT SUMMARY

The δ1-Catenin phospho-regulation antibody sampler kit may be used to examine phosphorylation of δ1-Catenin at Tyr-96, Tyr-228, Tyr-280, Tyr-302, and Thr-916. The kit also includes a monoclonal antibody to monitor total expression levels of δ1-Catenin.