Catalog # MK6660

MeCP2 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

Application / Dilution
ELISA, WB, ICC, AB

Species Reactivity

Hu, Rt, Ms

$395


DATASHEET  

KIT SUMMARY

The MeCP2 phospho-regulation kit can be used to detect phosphorylation of Ser-80 and Ser-421 in MeCP2. The kit includes an anti-MeCP2 (C-terminus) antibody for comparison total MeCP2 expression relative to site-specific phosphorylation level. In addition, the kit includes blocking peptides for examining the specificity of the antibodies in western blot and immunocytochemistry.

Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).

Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with anti-MeCP2 (Ser-421) (MP4611) in the absence (left) or presence (right) of blocking peptide (MX4615). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

MP4611 Wang, A. et al. (2014) PloS one 9(4): e94394. doi: 10.1371 WB: rat hippocampal CP1

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
MeCP2 (C-terminus) Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms75
MeCP2 (Ser-80), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms75
MeCP2 (Ser-421), phospho-specific Rabbit pAb 50 μlWB, E, ICCHu, Rt, Ms75
MeCP2 (C-terminus) Peptide50 μg AB, E
phospho-MeCP2 (Ser-80) Peptide50 μgAB, E
phospho-MeCP2 (Ser-421) Peptide 50 μgAB, E