Catalog # MK6050

MAP Kinase Activation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases that include three major MAPKs: p38, JNK, and ERK. These MAPKs are involved in many cellular programs such as cell proliferation, differentiation, motility, and death. Upon stimulation, the MAPKs are activated through a sequential protein kinase cascade consisting of MAPKKKs activating MAPKKs followed by dual phosphorylation and activation of MAPKs. p38 and JNK MAPKs participate in signaling cascades that control cellular responses to cytokines and stress. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, and MEF2. Active JNK dimers can translocate to the nucleus to regulate transcription through phosphorylation of c-Jun, ATF-2, and other transcription factors. The ERK1/2 (p44/42) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines. Activated ERKs have several downstream targets including p90RSK and Elk-1.

References
Davis, R.J. (1999) Biochem. Soc. Symp. 64:1.
Roux, P.P. & Blenis, J. (2004) Microbiol Mol Biol Rev 68:320.
Murphy, L.O. & Blenis, J. (2006) Trends Biochem Sci 31:268.
Owens, D.M. & Keyse, S.M. (2007) Oncogene 26:3203.

Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) then treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3 & 4).

Western blot analysis of human A431 cells untreated (lanes 1 & 4), treated with 100 nM calyculin A for 30 min. (lanes 2 & 5), or 100 ng/ml EGF for 60 min. (lanes 3 & 6). The blots were probed with anti-ERK1 (lanes 1, 2, & 3) or anti-ERK1/2 (Thr-202/Tyr-204) (lanes 4, 5, & 6).



Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

EM2331 Kyjacova, L. et al. (2015) Cell Death Differ. 22(6):898. WB: human DU145
EM2061 Park, K. et al. (2013) Mol Cell Biol. 33(4):752. WB: human keratinocytes
PM1391 Chambers, M.A. et al. (2009) J Physiol. 587:3363. WB: mouse muscle, C2C12
PM1391 Li, W. et al. (2009) AJP Cell Phys. 297:C706. WB: mouse C2C12 cells

This kit contains:

CATALOG#DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
ERK1 (C-terminal region) Mouse mAb50 μlWB, E, ICC, IHCHu, Rt, Ms44
ERK1 (Thr-202/Tyr-204)[conserved], phospho-specific Mouse mAb 50 μlWB, E, ICCHu, Rt, Ms44
JNK1 (C-terminal region) Mouse mAb 50 μlWB, E, ICC, IHCHu, Rt, Ms42
JNK (Thr-183/Tyr-185), phospho-specific Mouse mAb50 μlWB, E, ICCHu, Rt, Ms42
p38 (C-terminal) Mouse mAb50 μlWB, E, ICCHu, Rt, Ms38
p38 MAP Kinase (Thr-180/Tyr-182)), phospho-specific Mouse mAb50 μlWB, E, ICCHu, Rt, Ms28
KIT SUMMARY

The MAP kinase activation antibody sampler kit can be used to examine the activation of three major MAPKs: p38, ERK, and JNK. The kit also includes antibodies to monitor total expression levels of p38, ERK1/2, and JNK1.