Catalog # PK6580
Plexin A1, B1, and D1 Receptors Antibody Kit
Antibody Sampler Kit
| ELISA, WB, IP, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms |
 |
KIT SUMMARY The Plexin Receptor kit can be used to monitor the expression of Plexin A1, Plexin B1, and Plexin D1. The kit includes three antibodies for detecting different regions of Plexin D1, two antibodies to different regions in the Sema domain of Plexin A1, and an antibody that detects the C-terminal region of Plexin B1. |
Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6).
Western blots showing Cos7 cells transfected with Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (PP1301; lane 5). These blots were probed with anti-Plexin-A1 (PP1301; lanes 1 & 2) or with anti-Myc (lanes 3-5).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
PP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. (WB: rat brain)PP1841 Damola, A. et al. (2013) Prostate 73(12):1326. (WB, IP: Prostate Cells, shRNA knockdown)
PP1841 Soong, J. & Scott, G. (2013) J Cell Sci. 126(Pt 2):688. (WB: human melanocytes)
PP1841 Soong, J. et al (2012) J Invest Dermatol. 132(4):1230. (WB: melanocyte, keratinocyte, smooth muscle)
This kit contains:
| CATALOG # | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Plexin A1 (Sema Domain) Rabbit pAb | 50 μl | WB, E, IP, ICC, IHC | Hu, Rt, Ms | 220 | |
| Plexin A1 (Sema Domain) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 220 | |
| Plexin B1 (C-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 300 | |
| Plexin D1 Cytoplasmic domain) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 250 | |
| Plexin D1 (Sema domain) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 250 | |
| Plexin D1 (a.a. 1635-1647) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 250 |
