Catalog # AK6670
Arp2/3 Complex Antibody Kit
Antibody Sampler Kit
| ELISA, WB, IP, ICC |
Species Reactivity
| Hu, Rt, Ms, Ck, Fr |
 |
KIT SUMMARY The Arp2/3 complex kit can be used to detect expression levels of Arp2, Arp3, and Arpc1b in many different species. In addition, phospho-specific antibodies are included to detect phosphorylation of Arp2 (Thr-237/Thr-238) and Arpc1b (Thr-21). |
Western blot of Arpc1b phosphorylation in A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).
Immunocytochemical labeling of Arp2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with Arp2 (C-terminal region) and Arp2 (Thr-237/Thr-238) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to Cy3.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
AP3871 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophagesAP4351 Kim, Y.B. et al. (2015) J Biol Chem. 290(34):21076 WB: PC3 cells
AP3861 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophages
AP4321 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanoma
AP4351 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanoma
AP3861 Spillane, M. et al. (2012) J Neurosci. 32(49):17671. ICC: Chicken embryo neuron
AP3871 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cells
AP3861 Kalwa, H. & Michel, T. (2011) J Biol Chem. 286(3):2320. WB: bovine aortic endothelial cells
AP3861 Spillane, M. et al. (2011) Dev Neurobiol. 71(9):747. ICC: chick dorsal root ganglia
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Arp2 (C-terminal region) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck, Fr | 44 | |
| Arp2 (Thr-237/Thr-238), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 44 | |
| Arp3 (C-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, F | 47 | |
| Arpc1b/p41-Arc (C-terminal region) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms, Ck | 41 | |
| Arpc1b/p41-Arc (Thr-21), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms, Ck, F | 41 |
