Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.
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The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:AM2021 Dutta, P et al. (2014) J Neurochem. 130(3):360 WB: rat brain
AM2021 Muirhead, G et al. (2014) J Mol Neurosci. 53(1):125 WB: rat brain
AM2021 Pritchard, AJ et al. (2014) PLoS One. 9(6):e99444 WB: mouse splenocytes
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
AP1671 Vonach, C. et al. (2011) British J Cancer. 105:263. WB: human endothelial cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|Actin (N-terminal region) Rabbit pAb||50 μl||WB, E, IP, ICC, IHC||Hu, Rt, Ms, Ck||42|
|Actin (Tyr-53), phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck||42|
|Actin (C-terminal region) Mouse mAb||50 μl||WB, E, ICC, IHC||Hu, Rt, Ms, Ck||42|
|Anti-Mouse Ig:HRP Donkey pAb||100 μl||WB, E||Ms|
|Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The actin phospho-regulation kit can be used to examine phosphorylation of Ty-53 compared to the total expression of actin. The kit includes rabbit polyconal and mouse monoclonal antibodies to detect total actin, and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.