Catalog # NK6450
iNOS Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb |
 |
KIT SUMMARY The inducible nitric oxide synthase (iNOS) phospho-regulation antibody sampler kit can be used to examine Tyr-1055 phosphorylation in iNOS. The kit also includes a mouse monoclonal and rabbit polyclonal antibody to monitor total expression levels of iNOS, as well as HRP-conjugated secondary reagents. |
Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1µg/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NP2131).
Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1μg/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3, & 5). The blots were then treated with alkaline phosphatase (lanes 2, 4, & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), or mouse monoclonal anti-iNOS (lanes 5 & 6).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsMS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| iNOS(C-terminal region) Mouse mAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 130 | |
| iNOS (C-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 130 | |
| iNOS (Tyr-1055)[conserved site], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 130 | |
| Anti-Mouse Ig:HRPDonkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
