Catalog # CK6200

CRMP2 Phospho-Regulation Antibody Kit

Antibody Sampler Kit

DATASHEET  




$395

CRMP2 (CRMP-62, TOAD-64, DRP-2) is a microtubule associated protein involved in neuron development and axon pathfinding. CRMP2 binds to tubulin heterodimers and promotes microtubule assembly. The overexpression of CRMP2 facilitates the rate of axonal growth, whereas the mutated form that lacks activity toward the microtubule assembly inhibits axonal growth in a dominant negative manner. Phosphorylation of CRMP2 regulates its activity and this type of regulation has been implicated in axon growth cone collapse induced by several repulsive cues. Cdk5 and GSK3 phosphorylation occurs downstream of the repulsive cue, Sema-3A. Several residues in CRMP2 are phosphorylated by GSK3 (Ser-518, Thr-514, and Thr-509), and a priming site (Ser-522). These sites are conserved in human CRMP1 and CRMP4, but not in CRMP3 or CRMP5. The priming site is also phosphorylated by Cdk5. In contrast, ROCK phosphorylates Thr-555 leading to LPA, MAG, or Ephrin-A5 mediated growth cone collapse. Thus, CRMP2 phosphorylation status may be a critical element of pathways that control axon pathfinding. 

References
Arimura, N. et al. (2005) Mol. Cell. Biol. 25(22):9973.
Uchida, Y. et al. (2005) Genes to Cells 10:165.
Cole, A.R. et al. (2006) J. Biol. Chem. 281(24):16591.
Mimura, F. et al. (2006) J. Biol. Chem. 281(23):15970.

Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).

Immunocytochemical labeling of phosphorylated CRMP2 in mouse C2C12 cells. The cells were probed with CRMP2 (C-terminal region) and CRMP2 (Thr-555) rabbit polyclonal antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide (CX2165 or CX2255).



Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The peptides are supplied in PBS and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.



*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Product References:

CP2191 Sebastian, Q. et al. (2016) AIDS Res Hum Retrovir. 32(1):68 WB: human peripheral blood mononuclear cells
CP2191 Watamura, N. et al. (2016) J Neurosci Res. 94(1):15 WB: mouse brain
CP2191 Moutal, A. et al. (2015) Mol Neurobiol. :1 WB: rat primary cortical neurons
CP2191 Wilson, S.M. et al. (2014) Front Cell Neurosci. 28(8):135 WB: rat cortical neurons
CP2191 Wilson, S.M. et al. (2014) Front Cell Neurosci. 24(8):196 WB: rat cortical neurons
CP2191 Akama, K. et al. (2013) Biochim Biophys Acta. 1834(2):601. WB: monkey ES, NSS, NS, and AstP cells
CP2191 Qin, Z. et al. (2013) PLoS ONE 8(11):e80758. WB: mouse brain
CP2191 Yonedaa, A. et al. (2012) Mol Cell Biol. 32(10):1788. WB: rat fibroblasts, MDA-MB-231, SW480, MDCK
CP2251 Yonedaa, A. et al. (2012) Mol Cell Biol. 32(10):1788. WB: rat fibroblasts, MDA-MB-231, SW480, MDCK
CP2161 Brittain, J.M. et al. (2012) FEBS Letters 586(21):3813. WB: rat brain
CP2191 Namekata, K. et al. (2012) J Neurosci. 32(1):264. WB: COS7
CP2251 Crews, L. et al. (2011) Mol Neurodegener. 6:67. WB: rat neurons; human, rat, and mouse brain
CP2161 Foger, N. et al. (2011) J Exp Med. 208(9):1777. WB: mouse thymus, lymph node, spleen
CP2161 Lin, P.C. et al. (2011) J Biol Chem 286:41466-41478. WB: COS7, OLDN-93, NIH3T3, and N1E-115 cells
CP2191 Lim, Y-W et al. (2010) Bioch Biophys Res Comm 395(2):207. WB: rat cortical neurons

This kit contains:

CATALOG #DESCRIPTIONSIZEAPPLICATIONSSPECIES REACTIVITYMW (kDa)
CRMP2 (C-terminal region) Rabbit pAb50 μlWB, E, IP, ICCHu, Rt, Ms70
CRMP2 (Ser-522), phospho-specific Rabbit pAb50 μlWB, E, ICCHu, Rt, Ms70
CRMP2 (Thr-555), phospho-specific Rabbit pAb 50 μlWB, E, ICCHu70
CRMP2 (C-terminal region) Peptide50 μg AB, E
phospho-CRMP2 (Ser-522) Peptide50 μgAB, E
phospho-CRMP2 (Thr-555) Peptide 50 μgAB, E
KIT SUMMARY

The CRMP2 phospho-regulation antibody sampler kit can be used to detect the level of CRMP2 phosphorylation at Ser-522 and Thr-555 relative to the total expression level of CRMP2. The kit also includes peptides for antibody blocking experiments.