Catalog # MK6490
Myosin Light Chain Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb, Ck, Cw |
 |
KIT SUMMARY The myosin regulatory light chain (MLC) phospho-regulation antibody sampler kit can be used to examine phosphorylation of MLC at Ser-1 and Ser-19. The kit also includes a polyclonal antibody to monitor total expression levels of MLC, as well as secondary reagent for antibody detection. |
Western blot analysis of C2C12 cells untreated (lanes 1, 3, 5, & 7) or treated with Lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with monoclonal anti-MLC20 (clone MY-21) (lanes 1 & 2), polyclonal anti-MLC (Ser-19) phosho-specific (lanes 3 & 4), anti-MLC (Ser-1) phosho-specific (lanes 5 & 6), or anti-MLC (a.a. 11-22) (lanes 7 & 8).
Immunocytochemical labeling of phosphorylated MLC in paraformaldehyde fixed A7r5 cells. The cells were dual-labeled with anti-MLC (MM3441; middle) and anti-MLC (MP4201; top left), anti-MLC (Ser-19) (MP4221; middle left) and anti-MLC (Ser-1) (MP3461; bottom left). Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594 were used for detection of primary antibodies. The overlay of staining patterns are shown to the right.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
Customer Reviews
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
MP4221 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanomaRS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MP3461 Aguilar, H.N. et al. (2011) PLoS ONE 6(6):e20903. WB: human uterine myocytes
MP3461 Beach, J.R. et al. (2011) BMC Cell Biol. 12:52. ICC: PHK and HeLa cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Myosin Light Chain (a.a. 11-22) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 20 | |
| Myosin Light Chain (Ser-1), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Cw | 20 | |
| Myosin Light Chain (Ser-19), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck | 20 | |
| Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
