Both smooth muscle and nonmuscle myosin II activity is regulated by the phosphorylation state of the myosin regulatory light chain (MLC, MRLC, MLC20, Myl9). Phosphorylation of MLC at Thr-18 and Ser-19 activates myosin II motor activity and increases myosin filament stability. This activation has important roles in various cell motile processes. By contrast, other phosphorylation sites on MLC may inhibit myosin II activity. PKC phosphorylates Ser-1/Ser-2 and Thr-9 in MLC, and this phosphorylation decreases activated myosin II interaction with actin, as well as inhibits MLC interaction with the activation site kinase, myosin light-chain kinase. The Ser-1/Ser-2 region may be the major inhibitory site since Ser-1 is phosphorylated during PDGF-induced stress fiber disassembly and expression of unphosphorylatable MLC20 at the Ser-1/Ser-2 site suppresses this disassembly. Thus, inhibition of myosin II activity through phosphorylation of Ser-1/Ser-2 may have important roles in growth factor-induced reorganization of actomyosin filaments.
Tan, J.L. et al. (1992) Annu. Rev. Biochem. 61:721.
Sellers, J.R. (1991) Curr. Opin. Cell Biol. 3:98.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:MP4221 Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanoma
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
MP3461 Aguilar, H.N. et al. (2011) PLoS ONE 6(6):e20903. WB: human uterine myocytes
MP3461 Beach, J.R. et al. (2011) BMC Cell Biol. 12:52. ICC: PHK and HeLa cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
|CATALOG#||DESCRIPTION||SIZE||APPLICATIONS||SPECIES REACTIVITY||MW (kDa)|
|Myosin Light Chain (a.a. 11-22) Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck||20|
|Myosin Light Chain (Ser-1), phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Cw||20|
|Myosin Light Chain (Ser-19), phospho-specific Rabbit pAb||50 μl||WB, E, ICC||Hu, Rt, Ms, Ck||20|
|Anti-Rabbit Ig Light ChainSpecific:HRP Mouse mAb||100 μl||WB, E, ICC, IHC||Rb|
The myosin regulatory light chain (MLC) phospho-regulation antibody sampler kit can be used to examine phosphorylation of MLC at Ser-1 and Ser-19. The kit also includes a polyclonal antibody to monitor total expression levels of MLC, as well as secondary reagent for antibody detection.