Catalog # RK6100
Robo1 & Robo2 Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC, AB |
Species Reactivity
| Hu, Rt, Ms, Rb, Ck |
 |
KIT SUMMARY The Robo antibody sampler kit can be used to detect the level of Robo1 and Robo2 expression. The kit also includes peptides for antibody blocking experiments and a secondary reagent for antibody detection. |
Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-Robo1 (C-terminal region) in absence (lanes 1-3) or presence of Robo1 (C-terminal region) blocking peptide (RX2795; lanes 4-6).
Western blot analysis of C2C12 and SYF mouse cell lines. The blots were probed with anti-Robo2 (C-terminal region) in the absence (-) or presence (+) of Robo2 (C-terminal region) blocking peptide (RX2865).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| Robo1 (C-terminal region) Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 180 | |
| Robo1 (C-terminal region) Peptide | 50 μg | AB, E | |||
| Robo2 (C-terminal region) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms, Ck | 175 | |
| Robo2 (C-terminal region) Peptide | 50 μg | AB, E | |||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
