Catalog # PK6290
PTP1B Phospho-Regulation Antibody Kit
Antibody Sampler Kit
| ELISA, WB, ICC, IHC |
Species Reactivity
| Hu, Rt, Ms, Rb |
 |
KIT SUMMARY The PTP1B phospho-regulation antibody sampler kit can be used to detect phosphorylation of PTP1B at Ser-50 and Tyr-152. The kit also includes an antibody to examine total PTP1B expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection. |
Western blot image of mouse SYF cSrc-transformed cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with pervanadate (1 mM for 30 min.). The blots were probed with rabbit polyclonal anti-PTP1B (a.a. 146-157) (lanes 1 & 2) or anti-PTP1B (Tyr-152) (lanes 3 & 4).
Western blot image of human Jurkat cells untreated (lanes 1 & 3) or treated (lanes 2 & 4) with calyculin A (100 nM for 30 min.). The blots were probed with monoclonal anti-PTP1B (lanes 1 & 2) or polyclonal anti-PTP1B (Ser-50) (lanes 3 & 4).
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
Product References:
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblastsPP2351 Obanda, D.N. et al. (2012) Diabetes 61(3):597. WB: L6 myoblasts
PP2411 Obanda, D.N. et al. (2012) Diabetes 61(3):597. WB: L6 myoblasts
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
| PTP1B (C-terminal region) Mouse mAb | 50 μl | WB, E, ICC | Hu | 50 | |
| PTP1B (a.a. 146-157) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 50 | |
| PTP1B (Ser-50), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 50 | |
| PTP1B (Tyr-152), phospho-specific Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 50 | |
| Anti-Mouse Ig:HRP Donkey pAb | 100 μl | WB, E | Ms | ||
| Anti-Rabbit Ig Light-Chain Specific:HRP Mouse mAb | 100 μl | WB, E, ICC, IHC | Rb |
