Catalog # SX1715

Slingshot-1L (C-terminal region) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.

References

Soosairajah, J. et al. (2005) EMBO J. 24(3):473.
Endo, M. et al. (2003) J Neurosci. 23(7):2527.
Niwa, R. et al. (2002) Cell 108:233.

Western blot of rat PC12 cells. Blot was probed with anti-SSH1L (SP1711) in the absence (lane 1) or presence (lane 2) of SSH1L blocking peptide (SX1715).

A synthetic peptide corresponding to amino acid residues in the C-terminal region of human SSH1L. This sequence has 100% homology with similar regions of mouse and bovine SSH1L, and has low homology to SSH2L and SSH3L.

*For more information, see UniProt Accession Q8WYL5

Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-SSH1L (C-terminal region) antibody (SP1711) in ELISA, and has been shown to block the reactivity of SP1711 during Western blot. In addition, the peptide is recommended for use in blocking SP1711 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: