Catalog # JX3995

JMY (C-terminal region) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

JMY (junction mediating and regulatory protein) is a transcription co-factor, originally identified as a p300-binding protein involved in p53-dependent transcription. Upon DNA damage, JMY is released from Mdm2 inhibition and forms a complex with Strap and p300. This complex recruits PRMT5 to activate the p53 response. Through regulation of p53-dependent transcription, JMY has important roles in the DNA damage response. In addition, JMY contains three carboxyl-terminal WH2 actin binding domains which are are commonly found in WASP family proteins. JMY can bind to actin and to the Arp2/3 complex, as well as direct the assembly of actin filaments in vitro. These actin-regulating effects of JMY may have important roles in cell migration. When slow migrating HL-60 cells are differentiated into highly motile neutrophil-like cells, JMY moves from the nucleus to the cytoplasm and is concentrated at the actin-rich leading edge of cells. The loss of JMY leads to decreased cell migration in HL-60 cells. Thus, JMY represents a new class of multifunctional actin assembly factors whose activity may be regulated by cellular localization.

 

References
Coutts, A.S. et al. (2009) PNAS 106(47):19872.
Zuchero, J.B. et al. (2009) Nat Cell Biol. 11(4):451.
Shikama, N. et al. (1999) Molecular Cell 4:365.


JMY synthetic peptide corresponds to amino acids in the C-terminal region of mouse JMY. This sequence is highly conserved in human, rat, frog, and zebrafish JMY proteins, and has low homology to other WASP family members.

*For more information, see UniProt Accession Q9QXM1
Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-JMY (C-terminal region) antibody (JP3991) in ELISA, and has been shown to block the reactivity of JP3991 during Western blotting. In addition, the peptide is recommended for use in blocking JP3991 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY