Catalog # RX2075


C-Raf (C-terminus)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation.

 

References

Zhu, J. et al. (2005) Mol. Biol. Cell 16:4733.
Mason, C.S. et al. (1999) EMBOJ 18(8):2137.

C-Raf (C-terminus) synthetic peptide corresponding to amino acids 637 to 648 in human C-Raf. This sequence is conserved in C-Rafs from many species, and is not found in A-Raf or B-Raf.

*For more information, see UniProt Accession P04049
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-C-Raf (C-terminus) antibody (RP2071) in ELISA, and has been shown to block the reactivity of RP2071 during Western blot. In addition, the peptide is recommended for use in blocking RP2071 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of GST fusion protein containing human C-Raf. The blot was probed with polyclonal anti-C-Raf (C-terminus) antibody in the presence (lanes 2-4) or absence (lane 1) of C-Raf (C-terminus) blocking peptide (lane 2), C-Raf (Ser-471) peptide (lane 3), or an unrelated peptide (lane 4).



This kit contains:

KIT SUMMARY