Catalog # MX4575

Myosin 13/Extraocular Myosin (Hinge region) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).

 

References

Moncman, C.L. et al. (2011) Invest Ophthalmol Vis Sci. 52(7):3962.
Briggs, M.M. & Schachat, F. (2002). J Exp. Biol. 205:3133.
Berg, J.S. et al. (2001) Mol Biol Cell. 12:780.



A synthetic peptide corresponding to amino acid residues in the hinge region from mouse myosin-13/extraocular myosin. This sequence has 100% identity with rat and human myosin-13, and has low homology to other myosin family members.

*For more information, see UniProt Accession B1AR69
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-MYH13/Extraocular Myosin (Hinge region) antibody (MP4571) in ELISA, and has been shown to block the reactivity of MP4571 during Western blot. In addition, the peptide is recommended for use in blocking MP4571 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY