Catalog # EX1875

EGFR (a.a. 961-972) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173). These sites are important for downstream signaling and rapid internalization. In addition, EGFR activation leads to c-Src mediated phosphorylation of Tyr-845 and Tyr-1101. The former site is required for mitogenic responses to EGFR activation, while the latter may be an SH2 binding site. Phosphorylation of EGFR on serine and threonine residues is thought to represent a mechanism for regulation of receptor kinase activity and internalization. These sites include a PKC site (Thr-654), CAMKII sites (Ser-1046, 1047, 1057, and 1142), and constitutively phosphorylated sites (Ser-967 and Ser-1002). Thus, the regulation of EGFR activity involves a complex series of phosphorylation events at multiple sites throughout the intracellular portion of the receptor.

References

Morandell, S. et al. (2008) Proteomics. 8(21):4383.
Boeri Erba, E. et al. (2005) Mol. Cell. Prot. 4:1107.
Carpenter, G. (2000) Bioessays 22:697.

Immunocytochemical labeling in A431 cells untreated or treaed with Calyculin A (50 nM) for 15 min. The cells were labeled with anti-EGFR (a.a. 961-972) and anti-EGFR (Ser-967) polyclonal antibodies. The specificity of labeling was demonstrated for each antibody by blocking the signals with their respective blocking peptides, EGFR (a.a. 961-972) (EX1875) and phospho-EGFR (Ser-967) (EX1915).

EGFR synthetic peptide corresponding to amino acid residues 961-972 in human EGFR (ErbB-1). This human EGFR sequence has high homology to rat and mouse EGFR, and low homology to other ErbB family members.

*For more information, see UniProt Accession P00533

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by EGFR (a.a. 961-972) antibody (EP1871) in ELISA, and has been shown to block the reactivity of EP1871 in Western blot and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: