Catalog # CX4215

CXCR4 (a.a. 322-329) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKC and GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC and GRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylation of CXCR4 may be regulated by multiple kinases lead to complex regulation of CXCR4 signaling.

 

References

Busillo, J. M. et al. (2010) J Biol Chem. 285(10):7805.
Bhandari, D. et al. (2009) Mol Biol Cell. 20:1324.
Orsini, M.J. et al. (2000) J Biol Chem. 275(33):25876.
Orsini, M.J. et al. (1999) J Biol Chem. 274(43):31076.



CXCR4 (a.a. 322-329) synthetic peptide corresponds to amino acids in the C-terminal region of human CXCR4. This sequence is conserved in mouse, rat, and chicken CXCR4, but has low homology to other CXCR family members.

*For more information, see UniProt Accession P61073
Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-CXCR4 (a.a. 322-329) antibody (CP4211) in ELISA, and has been shown to block the reactivity of CP4211 during Western blot. In addition, the peptide is recommended for use in blocking CP4211 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY