Catalog # EX5125

EB3 (a.a. 171-182) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 protein stability during mitosis.

References

Komarova, Y. et al. (2012) Mol Cell. 48:914.
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1.
Komarova, Y. et al. (2005) Mol Biol Cell. 16(11):5334.

EB3 (a.a. 171-182) synthetic peptide corresponding to amino acid residues 171 to 182 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3, and has low homology to other EB family members.

*For more information, see UniProt Accession Q9UPY8

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-EB3 (a.a. 171-182) antibody (EP5121) in ELISA, and has been shown to block the reactivity of EP5121 in Western blot. In addition, the peptide is recommended for use in blocking EP5121 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: