Catalog # RX2015


B-Raf (N-terminus)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf(S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many phosphorylation sites including Ser-338, Tyr-341, and multiple catalytic domain sites. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity. Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.

 

References

Brummer, T. et al. (2006) Oncogene 25(47):6262.
Karbowniczek, M. et al. (2006) J Biol Chem 281(35):25447.
Wilhelm, S.M. et al. (2004) Cancer Res 64:7099.
Mason, C.S. et al. (1999) EMBOJ 18(8):2137.

A synthetic peptide corresponding to amino acid residues in the N-terminus of human B-Raf. This sequence has high homology with similar regions in rat and mouse B-Raf, and has low homology to other Raf family members.

*For more information, see UniProt Accession P15056
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-B-Raf (N-terminus) antibody (RP2011) in ELISA, and has been shown to block the reactivity of RP2011 during Western blot. In addition, the peptide is recommended for use in blocking RP2011 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY