Catalog # NX2115

Neuropilin-1 (a1 CUB Domain) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Neuropilins are transmembrane proteins that contain two CUB domains (a1 and a2), two coagulation factor-like domains (b1 and b2), and a MAM domain in the extracellular region. These proteins have short cytoplasmic domains that include a PDZ-binding motif. The neuropilin (NRP) family includes NRP-1, NRP-2A, and NRP-2B. NRP-1 has been implicated as a receptor involved in axon guidance and VEGF signaling. NRP-1 mediates activation of intracellular signaling pathways through interaction with its co-receptors, Plexin-A1 and VEGFRs. The expression of NRP-1, along with the co-receptor Plexin-A1, is upregulated in neurons after central nervous system injury. The axons from these neurons cannot cross semaphorin 3A-containing regions at the site of injury. Thus, semaphorin 3A and its co-receptors, Plexin-A1 and Neuropilin-1, may have significant roles in axon regeneration after neuronal injury.

 

References
Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.
Takahashi, T. et al. (1999) Cell 99:59.
He, Z. & Tessier-Lavigne, M. (1997) Cell 90:739.

Immunocytochemical double labeling using anti-HA mouse monoclonal and anti-NRP-1 rabbit polyclonal (NP2111) antibodies in COS-7 cells mock transfected (A,D) or transfected with NRP-1 constructs (B,E). The specificity of the binding in E was demonstrated by using NRP-1 peptide (NX2115) in the presence of the anti-NRP-1 antibody (C,F).



Neuropilin-1 synthetic peptide corresponds to amino acids within the a1 CUB domain of human neuropilin-1. This sequence is highly conserved in rat and mouse neuropilin-1, and has low homology to neuropilin-2.

*For more information, see UniProt Accession O14786
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-Neuropilin 1 (a1 CUB domain) antibody (NP2111) in ELISA, and has been shown to block the reactivity of NP2111 in Western blot and immunocytochemistry applications.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY