Catalog # SX1375

phospho-c-Src (Tyr-215) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed of nine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. The activity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation. Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 downregulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.

References

Vadlamudi, R.K. et al. (2003) FEBS Letters 543:76.
Obergfell, A. et al. (2002) J Cell Biol. 157(2):265.
Stover, D.R. et al. (1996) J Biol Chem 271(21):12481.

Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (20 µg/lane). The Blot was probed with anti-c-Src (Tyr-215) in the presence of no blocking peptide (lane 1), phospho-c-Src (Tyr-215) peptide (lane 2), unrelated phospho-β-Catenin (Tyr-142) peptide (lane 3), or BSA conjugated to phospho-tyrosine (lane 4). Peptides and BSA were used at 1 µg/ml.

Phospho-c-Src (Tyr-215) synthetic peptide corresponds to amino acid residues around tyrosine 215 of human c-Src. This peptide sequence has homology to other Src kinase family members, such as hck, fyn, csk, and yes.

*For more information, see UniProt Accession P12931

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-c-Src (Tyr-215) phospho-specific antibody (SP1371) in ELISA, and has been shown to block the reactivity of SP1371 during Western blot. In addition, the peptide is recommended for use in blocking SP1371 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: