Catalog # RX2865
Robo2 (C-terminal region) Peptide
Blocking Peptide
| Blocking | 1:1000 |
| ELISA | 50 ng/well |
Size 50 μg
The Robo family of repulsive guidance receptors (Robo1-4) have important roles in controlling axon guidance and cell migration. These receptors are members of the immunoglobulin (Ig) superfamily and consist of an ectodomain with five Ig domains and three fibronectin type III repeats, a single transmembrane domain, and a long cytoplasmic tail that contains four blocks of conserved cytoplasmic sequences. In Drosophila, mutations in Robo, and its midline-expressed ligand Slit, result in too many axons crossing and staying at the midline. Several proteins that regulate the actin cytoskeleton, including cAbl, Ena, and Rho-family GTPases, contribute to the Robo signaling pathway. cAbl phosphorylates Robo1 at Tyr-1073, and this may inhibit Robo activity, while Slit-Robo signaling activates both Rac and Rho, and inactivates Cdc42. Thus, Robo guidance receptors control axon outgrowth and cell migration through activation of cell signaling pathways that regulate cytoskeletal dynamics.
References
Bashaw, G.J. et al. (2000). Cell 101:703.
Yang, L. & Bashaw, G.J. (2006). Neuron 52:595.
Western blot analysis of C2C12 and SYF mouse cell lines. The blots were probed with anti-Robo2 (C-terminal region) in the absence (-) or presence (+) of Robo2 (C-terminal region) blocking peptide (RX2865).
*For more information, see UniProt Accession Q9HCK4
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains:
