Catalog # PX1435

phospho-PDK1 (Tyr-9) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survival kinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs. PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstream kinases, like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cell survival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid second messengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation is required for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylation may also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 are important for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.

References

Scheid, M.P. et al. (2005) Mol. Cell. Biol. 25(6):2347.
Taniyama, Y. et al. (2003) Mol Cell. Biol. 23(22):8019.
Park, J. et al. (2001) J Biol. Chem. 276(40):37459.

Western blot image of A431 cells untreated (lanes 1 and 3) or treated with pervanadate (lanes 2, 4, 5 & 6). Blots were probed with anti-PDK1 (PP1411) or anti-PDK1 (Tyr-9) (PP1431). The latter was used in the presence of no peptide (lane 4), phospho-PDK1 (Tyr-9) peptide (lane 5), or an unrelated phosphotyrosine peptide (lane 6).

Phospho-PDK1 (Tyr-9) synthetic peptide corresponds to amino acids surrounding tyrosine 9 of human PDK1. This sequence is highly conserved in rat and mouse PDK1.

*For more information, see UniProt Accession O15530

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-PDK1 (Tyr-9) phospho-specific antibody (PP1431) in ELISA, and has been shown to block the reactivity of PP1431 during Western blot. In addition, the peptide is recommended for use in blocking PP1431 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: