Catalog # WX4005


WASH (C-terminal region)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

The Wiskott–Aldrich syndrome protein (WASP) family functions downstream of Rho GTPases to mediate the branched-actin network formation required for cytoskeletal remodeling, intracellular transport, and cell motility. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms (WAVE1, 2, and 3). Two new WASP subfamilies are WASH and WHAMM. WASH is an F-actin binding protein that can promote Arp2/3-mediated branch actin nucleation. The WASH protein contains two WASH homology domains, a proline-rich region, and a C-terminal VCA domain. WASH can interact with dynamin, FAM21, and tubulin, and WASH activity may be regulated downstream of Rho GTPases. WASH interaction with FAM21 has been implicated in endosome sorting, and inactivation of WASH leads to endosome tubulation. Thus, WASH may be a critical element in the regulation of the actin filament and microtubule networks that mediate endosomal sorting and fission.

 

References

Derivery, E. et al. (2009) Dev. Cell. 17:712.
Gomez, T.S. & Billadeau, D.D. (2009) Dev. Cell. 17:699.
Liu, R. et al. (2009) Development. 136:2849.
Linardopoulou, E.V. et al. (2007) PLoS Genet. 3:e237.

WASH synthetic peptide corresponds to amino acids in the C-terminal region of human WASH. This sequence is highly conserved in rat, mouse, and chicken WASH, and has low homology to other WASP family members.

*For more information, see UniProt Accession A8K0Z3
Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-WASH (C-terminal region) antibody (WP4001) in ELISA, and has been shown to block the reactivity of WP4001 during Western blotting. In addition, the peptide is recommended for use in blocking WP4001 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of human Jurkat cells (lanes 1-3). The blots were probed with anti-WASH (C-terminal region) rabbit polyclonal antibody at 1:250 (lane 1) or at 1:1000 in the absence (lane 2) or presence of WASH blocking peptide (WX4005) (lane 3).



This kit contains:

KIT SUMMARY