Catalog # WX2125

unphosphorylated-WAVE (Tyr-150) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

The Wiskott–Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an N-terminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.

 

References

Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.
Leng, Y. et al. (2005) PNAS 102(4):1098.
Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.
Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.



Unphosphorylated WAVE (Tyr-150) synthetic peptide corresponds to amino acid residues surrounding the Tyr-150 in human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and to similar regions in the conserved site in WAVE1 (Tyr-151) and WAVE3 (Tyr-151).

*For more information, see UniProt Accession Q9Y6W5
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This peptide can be used as an unphosphorylated control peptide for WAVE (Tyr-150) antibody (WP1821). It is the unphosphorylated version of phospho-WAVE (Tyr-150) peptide (WX1825), and it does not block WP1821 reactivity in Western blot, demonstrating the phospho-specificity of WP1821.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY