Catalog # VX2945

VEGFR-3 (a.a.1285-1298) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.

 

References

Garcia Blanes, M. et al. (2007) J Biol. Chem. 282(14):10660.
Kiselyov, A. et al. (2007) Expert Opin. Investig. Drugs 16(1):83.
Robinson, C.J. & Stringer, S.E. (2001) J. Cell Sci.114:853.
Meyer, M. et al. (1999) EMBO J. 18:363.
Dougher-Vermazen, M. et al. (1994) Bioch Biophys Res Com. 205:728.

Western blot image of human K-562 cells (lanes 1 & 2) and HUVEC (lanes 3 & 4). The blots was probed with rabbit polyclonal anti-VEGFR-3 (a.a. 1285-1298) in the absence (lanes 1 & 3) or presence of blocking peptide (VX2945) (lanes 2 & 4).



A synthetic peptide corresponding to amino acids 1285 to 1298 in human VEGFR-3. This sequence has two amino acid differences from rat VEGFR-3, and five amino acid differences from mouse VEGFR-3.

*For more information, see UniProt Accession P35916
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-VEGFR-3 (a.a. 1285-1298) antibody (VP2941) in ELISA, and has been shown to block the reactivity of VP2941 during Western blot. In addition, the peptide is recommended for use in blocking VP2941 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY