Catalog # VX2705

Vav3 (a.a. 293-305) Peptide

Blocking Peptide

Application / Dilution
ELISA50 ng/well

Size 50 μg



The Vav family of Rho-guanine nucleotide exchange factors, Vav1, Vav2, and Vav3, have central roles in transducing signals from cell surface receptors, such as integrin, growth factor and immune cell receptors to the cytoskeleton. This role includes receptor-mediated changes in the actin cytoskeleton and cell motility. Vav1 expression is normally restricted to hematopoietic cells, while Vav2 and Vav3 are more widely expressed. All three Vav isoforms have been shown to be abnormally expressed in several types of cancer. Vavs are composed of multiple domains, including a Dbl homology domain, a calponin homology domain, an acidic amino acid region, a pleckstrin homology domain, a cysteine-rich domain, and SH3 and SH2 domains. Vav activity is regulated by the phosphorylation status of several conserved tyrosine residues in the acidic region (In Vav2: Tyr-142, Tyr-159, and Tyr-172). These tyrosine residues are able to participate in autoinhibitory interactions with the Dbl homology domain and this interaction is prevented after phosphorylation of these sites leading to activation of Vav GEF activity.



Wilsbacher, J.L. et al. (2006) Cell Comm. Signal. 4:5.
Aghazadeh, B. (2000) Cell 102:625.
Movilla, N. et al. (1999) Mol. Cell. Biol. 19:7870.

A synthetic peptide corresponding to amino acid residues in the Dbl homology domain of human Vav3. This sequence has high homology with similar regions in rat and mouse Vav3, and has low homology to other Vav family members.

*For more information, see UniProt Accession Q9UKW4
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

This peptide is specifically recognized by Vav3 (a.a. 293-305) antibody (VP2701) in ELISA, and has been shown to block the reactivity of VP2701 in Western blot and is recommended for blocking in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: