Catalog # TX5675


phospho-TRPM7 (Ser-1513)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.

 

References

Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23.
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793

Phospho-TRPM7 (Ser-1513) synthetic peptide corresponding to amino acids surrounding Ser-1513 in human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.

*For more information, see UniProt Accession Q96QT4
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by TRPM7 (Ser-1513), antibody (TP5671) in ELISA, and has been shown to block the reactivity of TP5671 in Western blot. In addition, the peptide is recommended for use in blocking TP5671 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY