Catalog # TX4185

Talin (a.a. 420-429) Peptide

Blocking Peptide

Application / Dilution
ELISA50 ng/well

Size 50 μg



Talin is an important cytoskeletal component of integrin adhesion sites. Calpins cleave talin precursor (240 kDa) into an amino-terminal globular head domain of 47 kDa and a carboxyl-terminal 190 kDa rod domain. The talin head domain contains a FERM domain, that binds integrins, PIP kinase (Type I), and FAK. The rod domain has several vinculin-binding sites, a second integrin-binding site, and two actin-binding sites. These talin protein-protein interactions are critical for integrin activation, focal adhesion formation and cell migration. Talin regulation may occur through phosphorylation and regulated degradation. The talin head domain binds Smurf1, an E3 ubiquitin ligase, and this interaction leads to talin head ubiquitylation and degradation. Cdk5 can phosphorylate Ser-425 in the head domain, and this inhibits both binding to Smurf1 and subsequent degradation. The S425A talin mutant resists Cdk5 phosphorylation, increases susceptibility to Smurf1-mediated ubiquitylation, and inhibits cell migration. Thus, talin head phosphorylation may be important for regulating adhesion stability and cell migration.



Huang, C. et al. (2009) Nat Cell Biol. 11(5):624.
Moser, M. et al. (2009) Science. 324(5929):895.
Ratnikov, B. et al. (2005) J. Cell Sci. 118:4921.

Talin (a.a. 420-429) synthetic peptide corresponding to amino acids 420 to 429 in human Talin 1. This sequence is conserved in rat, mouse, and chicken Talin 1, as well as in Talin 2.

*For more information, see UniProt Accession Q9Y490
Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is recommended for use in ELISA and antibody-blocking experiments in Western blot and immunocytochemistry applications.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: