Catalog # SX4065

nSMase2 (C-terminal region) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependance. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSmase2 (also known as SMPD3) is expressed primarily in brain, and co-localizes with Golgi markers in several cell lines. The activity and phosphorylation of nSmase2 is upregulated in response to oxidative stress. In addition, nSMase-2 binds calcineurin, and this interaction inhibits nSMase2 activity.

References

Filosto, S. et al. (2010) J Biol Chem. 285(14):10213.
Stoffel, W. et al. (2005) Proc Natl Acad Sci U S A. 102(12):4554.
Hofmann, K. et al. (2000) Proc Natl Acad Sci U S A. 97(11):5895.

Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or at 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).

nSMase2 synthetic peptide corresponds to amino acids in the C-terminal region of human nSMase2. This sequence is highly conserved in rat and mouse nSMase2, and has low homology to other nSMase family members.

*For more information, see UniProt Accession Q9NY59

Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-nSMase2 (C-terminal region) antibody (SP4061) in ELISA, and has been shown to block the reactivity of SP4061 during Western blot. In addition, the peptide is recommended for use in blocking SP4061 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: