Catalog # SX3845

SCAI (N-terminal region) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μl

DATASHEET  




$55

Cellular processes such as proliferation, differentiation, and cell migration are directed through the highly regulated control of transcriptional cofactors. During cell migration, serum response factor (SRF) is a central transcription factor that interacts with MAL to induce gene expression in response to actin assembly changes caused by the RhoA-mDia1 pathway. SCAI (suppressor of cancer cell invasion) is a highly conserved protein that is thought to regulate invasive cell migration and act on the RhoA–mDia1 signal transduction pathway. SCAI localizes in the nucleus, where it is thought to bind and inhibit MAL, by forming a ternary complex with SRF. Suppression of SCAI expression has been shown to upregulate β1-integrin expression, an intergrin that is also upregulated in several human malignancies. In addition, SCAI is downregulated in various human tumors, and decreased levels of SCAI correlate with increased invasive cell migration. Thus, SCAI is a transcriptional cofactor that may be important for regulating gene expression downstream of mDia1 to direct changes in cell motility.

 

References
Brandt, D.T. et al. (2009) Cell Cycle. 8(16):2523.
Brandt, D.T. et al. (2009) Nat Cell Biol. 11(5):557.

Western blot analysis of adult mouse brain. The blot was probed with rabbit polyclonal anti-SCAI (N-terminal region) antibody in the presence (lanes 2) or absence (lane 1) of SCAI (N-terminal region) blocking peptide (SX3845), or unrelated peptide (lane 3).



SCAI synthetic peptide corresponding to amino acid residues in the N-terminal region of mouse SCAI. This peptide sequence is highly conserved in human, rat, mouse, and chicken SCAI.

*For more information, see UniProt Accession Q8C8N2
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by SCAI (N-terminal) antibody (SP3841) in ELISA, and has been shown to block the reactivity of SP3841 in Western blot. In addition, the peptide is recommended for use in blocking SP3841 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY