Catalog # SX1535

phospho-SHP1 (Ser-591) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

SHP1 (PTP1C, SH-PTP1, or HCP) is a protein-tyrosine phosphatase (PTP) involved in cell migration, cell proliferation, and immune cell function. This phosphatase contains two N-terminal SH2 domains and a C-terminal phosphatase domain. SHP1 associates with a variety of cytokine and growth factor receptors and regulates signal transduction through dephosphorylation of these receptors or their downstream effectors. Downstream of receptor activation, SHP1 regulates the transcriptional activity stimulated by JAK/Stat and MAPK pathways. SHP1 activity is regulated by both tyrosine and serine phosphorylation. Phosphorylation of Tyr-536 and Tyr-564 stimulates phosphatase activity and promotes interaction with Grb-2. Serine phosphorylation at Ser-591 is mediated by PKCα and leads to inhibition of phosphatase activity. Thus, phosphorylation at tyrosine relative to serine residues may be regulated by different cell signaling pathways to control SHP1 activity.

References

Jones, M.L. et al. (2004) J. Biol. Chem. 279(39):40475.
Zhang, Z. et al. (2003) J. Biol. Chem. 278(7):4668.
Zhang, J. et al. (2000) Semin. Immunol. 12:361.

Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) and then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho-serine peptide (lane 6).

Phospho-SHP1 (Ser-591) synthetic peptide corresponds to amino acids around serine 591 of human SHP1. The sequence is highly conserved in rat and mouse SHP1.

*For more information, see UniProt Accession P29350

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-SHP1 (Ser-591) phospho-specific antibody (SP1531) in ELISA, and has been shown to block the reactivity of SP1531 during Western blot. In addition, the peptide is recommended for use in blocking SP1531 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: