Catalog # SX1485


phospho-Stat5 (Tyr-694)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr-694 is required for Stat5A activation. Stat5 has been found to be constitutively active in some leukemic cell types. Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility. Both Stat5A (Tyr-694) and Stat5B (Tyr-699) are independently regulated and activated in various cell types. For instance, both isoforms are activated in response to IFNα in B cells, but only Stat5A is phosphorylated in response to IFNα in HeLa cells.

 

References
Darnell, J.E. (1997) Science 277:1630.
Meinke, A. et al. (1996) Mol. Cell. Biol. 16:6937-6944.
Gouilleux, F. et al. (1994) EMBO J. 13:4361.
Wakao, H. et al. (1994) EMBO J. 13:2182.
Phospho-Stat5 (Tyr-694) synthetic peptide corresponds to amino acid residues around tyrosine 694 of human Stat5A. This peptide sequence is identical to tyrosine 699 in human Stat5B, and has high homology to the conserved tyrosine site in rat and mouse Stat5A/B.

*For more information, see UniProt Accession P42229
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-Stat5 (Tyr-694) phospho-specific antibody (SM1481) in ELISA, and has been shown to block the reactivity of SM1481 in Western blot. In addition, the peptide is recommended for use in blocking SM1481 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY