Catalog # SX1225

Semaphorin-3A (Central region) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

 

References

Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
Kolodkin, A.L. et al. (1993) Cell 75:1389.
Luo, Y. et al. (1993) Cell 75:217.



Semaphorin 3A synthetic peptide corresponds to amino acids in the central region of human Sema3A. This sequence is highly conserved in rat and mouse Sema3A, and has low homology to other semaphorin family members.

*For more information, see UniProt Accession Q14563
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-Sema3A (Central region) antibody (SP1221) in ELISA, and has been shown to block the reactivity of SP1221 during Western blot. In addition, the peptide is recommended for use in blocking SP1221 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY