Catalog # SX0285

nSMase3 (C-terminal region) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependence. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase3 (also known as SMPD4) is highly expressed in skeletal muscle and heart tissues, and is expressed in many cancer cell lines. The nSMase3 protein is localized to endoplasmic reticulum and Golgi, and may be upregulated in response to DNA damage and cell stress.

 

References

Corcoran, C.A. et al. (2008) Mol Can Res. 6(5):795.
Wiegmann, K.O. et al. (2006) J Biol Chem. 281(19):13784.



nSMase3 synthetic peptide corresponds to amino acids in the C-terminal region of mouse nSMase3. This sequence is highly conserved in rat and human nSMase3, and has low homology to other nSMase family members.

*For more information, see UniProt Accession Q6ZPR5
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by nSMase3 (C-terminal region) antibody (SP0281) in ELISA, and has been shown to block the reactivity of SP0281 in Western blot. In addition, the peptide is recommended for use in blocking SP0281 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY