Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependence. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase3 (also known as SMPD4) is highly expressed in skeletal muscle and heart tissues, and is expressed in many cancer cell lines. The nSMase3 protein is localized to endoplasmic reticulum and Golgi, and may be upregulated in response to DNA damage and cell stress.
Corcoran, C.A. et al. (2008) Mol Can Res. 6(5):795.
Wiegmann, K.O. et al. (2006) J Biol Chem. 281(19):13784.
*For more information, see UniProt Accession Q6ZPR5
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*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
This kit contains: