Catalog # PX4505

phospho-Paxillin (Tyr-118) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr-31 and Tyr-118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.

 

References

Schaller, M.D & Schaefer, E.M. (2001) Biochem J. 360:57.
Salgia, R. et al. (1995) J Biol Chem. 270(49):29145.



Phospho-Paxillin (Tyr-118) synthetic peptide corresponding to amino acid residues around tyrosine 118 from human paxillin. This sequence is highly conserved in rat and mouse paxillin and is also found in all isoforms (a, b, g) of paxillin.

*For more information, see UniProt Accession P49023
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-Paxillin (Tyr-118) phospho-specific antibody (PP4501) in ELISA, and has been shown to block the reactivity of PP4501 during Western blot. In addition, the peptide is recommended for use in blocking PP4501 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY