Catalog # PX3955


phospho-Prion Protein (Ser-43)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc). The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease.

 

References

Gianoupolous, P.N. et al. (2009) J. Neurosci. 29:8743.
Monari, L. et al. (1994) Proc. Natl. Acad. Sci. 91:2839.
Kascsak, R.J. et al. (1987) J. Virology. 61:3688.
Prusiner, S.B. (1982) Science. 216:136.

Phospho-Prion Protein (Ser-43) peptide includes amino acids surrounding serine 43 in human prion protein. This sequence has high homology to the conserved site in rat, mouse, and bovine prion protein.

*For more information, see UniProt Accession P04156
Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-Prion Protein (Ser-43) antibody (PP3951) in ELISA, and has been shown to block the reactivity of PP3951 during Western blot. In addition, the peptide is recommended for use in blocking PP3951 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.



This kit contains:

KIT SUMMARY