Catalog # PX1845


Plexin B1 (C-terminal region)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. In addition, Plexin B1 and B2 contain cleavage sites for subtilisin-like proprotein convertases. These cleavage sites may be important for regulating assembly of heterodimeric receptors or release of the extracellular domain. Plexin-B1 is expressed as a 300 kDa precursor protein that can be cleaved into a 200 kDa a-subunit and a 100 kDa b-subunit. This plexin is the receptor for the class IV semaphorin, Sema-4D. Sema-4D activation of Plexin B1 recruits and activates c-Met, which can promote cell survival, migration, and angiogenesis.

 

References

Basile, J.R. et al. (2006) PNAS 103(24):9017.
Artigiani, S. et al. (2003) J Biol. Chem. 278(12):10094.
Tamagnone, L. et al. (1999) Cell 99:71.

Plexin B1 (C-terminal) peptide includes amino acid residues in the C-terminal region of human Plexin B1. This sequence has high homology with similar regions in rat and mouse Plexin B1, and has low homology to other plexin family members.

*For more information, see UniProt Accession O43157
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by Plexin B1 (C-terminal region) antibody (PP1841) in ELISA, and has been shown to block the reactivity of PP1841 in Western blot and immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Western blot of human HepG2 cells. The blot was probed with anti-Plexin B1 in the presence (lane 2) or absence (lane 1) of Plexin B1 peptide.



This kit contains:

KIT SUMMARY