Catalog # PX1455

phospho-p130 Cas (Tyr-762) Peptide

Blocking Peptide

Application / Dilution

Blocking	1:1000
ELISA	50 ng/well

Size 50 μg

p130 Cas (Crk-associated substrate (CAS), breast cancer antiestrogen resistance 1 (BCAR1)) is a docking protein containing multiple protein-protein interaction domains. The N-terminal SH3 domain functions as a molecular switch regulating CAS tyrosine phosphorylation, as it interacts with tyrosine kinases and phosphatases. The C-terminal Src binding domain contains a proline-rich motif that mediates interaction with the SH3 domains of Src-family kinases (SFKs). Phosphorylation of this domain at Tyr-762 in rat (Tyr-668 in mouse) promotes this interaction. The p130 Cas central substrate domain is characterized by 15 tyrosines present in Tyr-X-X-Pro (YXXP) motifs, including Tyr-165, Tyr-249, and Tyr-410. When phosphorylated, most YXXP motifs are able to serve as docking sites for proteins with SH2 or PTB domains. In addition, phosphorylation of Tyr-751 (Tyr-653 in human) near the C-terminal caspase recognition site can attenuate caspase cleavage, while dephosphorylation occurs during apoptosis and may facilitate p130 Cas degradation.

References

Shin, N-Y. et al. (2004). J Biol Chem. 279(37):38331.
Kim, D.H. et al. (2003). Biochem Biophys Res Comm. 300:141.
Pellicena, P. & Miller, W.T. (2001) J Biol Chem. 276 (30):28190.
Nakamoto, T. et al. (1996) J Biol Chem. 271(15):8959.

Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4-6). The blot was probed with anti-p130 Cas (lanes 1 & 2) or anti-p130 Cas (Tyr-762) (lanes 3-6). The latter was used in the presence of no peptide (lane 4), phospho-p130 Cas (Tyr-762) peptide (lane 5) or phospho-p130 Cas (Tyr-751) peptide (lane 6).

Phospho-p130 Cas (Tyr-762) synthetic peptide corresponds to amino acid residues around tyrosine 762 in the Src-binding domain of rat p130 Cas. This rat sequence is the same as Tyr-664 in human p130 Cas and Tyr-664 in mouse p130 Cas. This site is also highly conserved in Cas-L (HEF1, Nedd9).

*For more information, see UniProt Accession Q63767

Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-p130 Cas (Tyr-762) phospho-specific antibody (PP1451) in ELISA, and has been shown to block the reactivity of PP1451 during Western blot. In addition, the peptide is recommended for use in blocking PP1451 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: