Catalog # NX4055

phospho-eNOS (Ser-1177) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes, inducible NOS (iNOS, NOS-II), neuronal NOS (bNOS, NOS-I), and endothelial NOS (eNOS, ecNOS, NOS-III). Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation. Tyr-657 is phosphorylated by PYK2 in response to fluid shear stress and this phosphorylation leads to attenuation of enzyme activity.

 

References

Loot, A. E. et al. (2009) J Exp Med. 206(13):2889.
Fisslthaler, B. et al. (2008) Circ Res. 102:1520.
Mount, P.F. et al. (2007) J Mo.l Cell. Cardiol. 42(2):271.
Musicki, B. et al. (2005) Proc. Natl. Acad. Sci.102(33):11870.



Phospho-eNOS (Ser-1177) synthetic peptide corresponds to amino acids surrounding Ser-1177 in human eNOS. This sequence is conserved in rat and mouse eNOS.

*For more information, see UniProt Accession P29474
Blocking peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-eNOS (Ser-1177) phospho-specific antibody (NP4051) in ELISA, and has been shown to block the reactivity of NP4051 during Western blot. In addition, the peptide is recommended for use in blocking NP4051 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY