phospho-Myosin IIA Heavy Chain (Ser-1916) Peptide

Non-muscle myosin II is an actin-based motor protein essential to cell motility, division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, which activates myosin II motor activity and promotes filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. Several kinases phosphorylation NMHC-II isoforms directly. TRPM7 phosphorylates Thr-1800, Ser-1803, and Ser-1808, which reduces NMHC-IIA incorporation into the actin cytoskeleton. PKC phosphorylates NMHC-IIA on Ser-1916 and NMHC-IIB on multiple tailpiece serines leading to inhibition of filament assembly. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 and increases disassembly of NMHC-IIA filaments.

References

*For more information, see UniProt Accession P35579

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.