Catalog # MX5185

phospho-Myosin IIA Heavy Chain (Ser-1803) Peptide

Blocking Peptide

Application / Dilution
ELISA50 ng/well

Size 50 μg



Non-muscle myosin II is an actin-based motor protein essential to cell motility, division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are three NMHC-II isoforms (NMHC-IIA, NMHC-IIB, and NMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation of NMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, which activates myosin II motor activity and promotes filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. Several kinases phosphorylation NMHC-II isoforms directly. TRPM7 phosphorylates Thr-1800, Ser-1803, and Ser-1808, which reduces NMHC-IIA incorporation into the actin cytoskeleton. PKC phosphorylates NMHC-IIA on Ser-1916 and NMHC-IIB on multiple tailpiece serines leading to inhibition of filament assembly. Casein kinase II phosphorylates NMHC-IIA on Ser-1943 and increases disassembly of NMHC-IIA filaments.



Dulyaninova, N. & Bresnick, A. (2013) BioArchitect. 3(4):1.
Breckenridge, M.T. et al. (2009) Mol Biol Cell 20:338.
Dulyaninova, N.G. et al. (2007) Mol Biol Cell 18:3144.
Murakami, N. et al. (1998) Biochemistry 37:1989.

Phospho-Myosin IIA Heavy Chain (Ser-1803) synthetic peptide corresponding to amino acid residues surrounding Ser-1803 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse myosin IIA heavy chain, and the site has some homology to the conserved sites in nonmuscle myosin IIB and smooth muscle myosin heavy chains.

*For more information, see UniProt Accession P35579
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by Myosin IIA Heavy Chain (Ser-1803) phospho-specific antibody (MP5181) in ELISA, and has been shown to block the reactivity of MP5181 in Western blot. In addition, the peptide is recommended for use in blocking MP5181 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains: