Catalog # MX4615


phospho-MeCP2 (Ser-421)

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

$55

Methyl-CpG Binding Protein 2 (MeCP2) was identified based on its affinity for methylated cytosines within DNA. As a chromatin-associated multifunctional protein, MeCP2 has been implicated in regulation of transcription and chromatin structure. Mutations of MeCP2 cause Rett syndrome, which results from neuronal dysfunction and impairment in cognitive and motor functions. Regulation of MeCP2 activity may involve phosphorylation at multiple sites. Ser-421 in MeCP2 is phosphorylated in response to neuronal activity, calcium influx, and is dependent on Cam-KII. Alanine mutation of Ser-421 leads to defects in synapse development and activity. Ser-80 in MeCP2 is phosphorylated in HeLa nuclear extracts and neurons. Alanine mutation of Ser-80 attenuates MeCP2 chromatin association and leads to locomotor deficits in transgenic knock-in mice. Thus, phosphorylation of MeCP2 may be important for altering its function during neuronal activity.

 

References

Cohen, S. et al. (2011) Neuron 72:72.
Tao, J. et al. (2009) PNAS 106(12):4882.
Lewis, J.D. et al. (1992). Cell 69:905.

phospho-MeCP2 (Ser-421) synthetic peptide corresponding to amino acid residues surrounding serine 421 in mouse MeCP2. This peptide sequence is highly conserved in rat and human MeCP2, and has low homology to other nuclear proteins.

*For more information, see UniProt Accession Q9Z2D6
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-MeCP2 (Ser-421) antibody (MP4611) in ELISA, and has been shown to block the reactivity of MP4611 during Western blot. In addition, the peptide is recommended for use in blocking MP4611 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-421) rabbit polyclonal antibody (MP4611) in the absence (left) or presence (right) of blocking peptide (MX4615). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.



This kit contains:

KIT SUMMARY