Catalog # MX4555

Myosin 7B/MHC14 (Hinge region) Peptide

Blocking Peptide

Application Dilution
Blocking1:1000
ELISA50 ng/well

Size 50 μg

DATASHEET  




$55

Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).

 

References

Rubio, M.D., et al. (2011) J Neurosci. 31(4):1448.
Rossi, A.C. et al. (2009) J Physiol. 588(Pt 2):353.
Berg, J.S. et al. (2001) Mol Biol Cell. 12:780.

Western blot analysis MYH7B in mouse brain (lane 1 & 2) and mouse extraocular muscle (lane 3 & 4). The blot was probed with rabbit polyclonal anti-MYH7B/MHC14 (Hinge region) at 1:500 in absence (lanes 1 & 3) and presence of MYH7B blocking peptide (MX4555; lanes 2 & 4).



A synthetic peptide corresponding to amino acid residues in the hinge region from mouse MYH7B/MHC14. This sequence is highly conserved in rat and human MYH7B, and has less than 50% identity with other MYH family members.

*For more information, see UniProt Accession A2AQP0
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.

The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.

The peptide is specifically recognized by anti-MYH7B/MHC14 (Hinge region) antibody (MP4551) in ELISA, and has been shown to block the reactivity of MP4551 during Western blot. In addition, the peptide is recommended for use in blocking MP4551 reactivity in immunocytochemistry.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.

This kit contains:

KIT SUMMARY